High resolution NMR spectroscopy for metabolomics and radiochemistry:
As only research group within the University Hospital, the WSIC is equipped with a high resolution 14.1 Tesla NMR spectrometer and three NMR probes for different tasks. A 5 mm triple resonance (1H, 13C, 15N) probe is mainly used by our chemists to assist tracer development and to perform standardized metabolomics clinical IVDr measurements of biofluids such as blood, urine and liquor. For the analysis of human and murine tissue, a 4 mm high-resolution magic angle spinning (HR-MAS) probe is available. Finally, a highly-sensitive 1.7 mm TXI (1H, 13C, 31P) microprobe can be used for the analysis of very mass- or volume-limited samples.
Ex-vivo measurements by high-resoluton NMR allow a much more detailed view into cellular mechanisms during cancer, neurodegenerative diseases or inflammation and can help to develop new biomarkers and tracers. Our Bruker 600 MHz Avance III HD system is hereby fully automated with a cooled autosampler and allows a high-throughput for large (pre)clinical studies. The NMR lab is further S2 certified and consists of additional instruments for the appropriate sample preparation, such as an focused ultrasonicator and a cryogenic tissue pulverizer.
For further information, please contact the NMR device manager, Dr. Christoph Trautwein.
