Multiscale Immunoimaging Workshop 10.-14. February 2025
Join us for an immersive and hands-on workshop to advance your skills in multiscale immunoimaging. We will provide an overview of state-of-the-art microscopic and macroscopic imaging modalities and their applications for visualizing the function of the immune system. Our workshop focuses on hands-on training rather than lectures. Each participant will work with multiple imaging modalities, making this event ideal for both experienced and new imaging professionals. The workshop is limited to 30 participants, so early registration is recommended.
Content
Topics
• Live-cell imaging of immune effector function
• Intravital multiphoton & higher harmonic generation microscopy
• Tissue clearing and light-sheet microscopy
• Functional MR for monitoring immunotherapy response
• PET/CT for immune cell tracking
• Correlating multiscale imaging modalities
Activities
• Learn from experts in each imaging modality
• Get hands-on experience in modern imaging techniques
• Listen to application examples from the bench and the clinic
• Learn how to extract data from images and how to correlate multimodal image data
• Get expert advice for your own experiments and image or analyze your own sample or data
• Explore datasets in virtual reality
• Test your new knowledge in a Pub Quiz
• Exchange knowledge and network with peers!
Program
In small groups, you will get hands-on experience on all imaging devices and learn about applications for imaging the immune system. In the evenings, we invite you to focus sessions for discussing your own samples or data with our experts or in depth practice in the imaging or analysis technique of your choice.
Monday
09:00 Welcome Breakfast & Introduction
10:00 ‘Hands-on theory’ at the microscope: Principles of live-cell imaging of immune cell dynamics
12:00 Lunch
13:00 Hands-on imaging: Live-cell imaging of immune cell effector function using optical reporters
15:00 Coffee Break
15:30 Image analysis: Automating quantification of immune cell function using ImageJ and deep learning tools
17:00 Break
17:30 Evening lecture: Multiscale immunoimaging to understand systemic immunotherapy response
18:30 Dinner on site
19:00 Hands-on focus sessions (voluntarily)
Tuesday
09:00 Morning lecture: Noninvasive imaging of immune function in the tumor microenvironment using PET/MR
10:00 ‘Hands-on theory’ at the microscope: Principles of multiphoton microscopy & higher harmonic generation
12:00 Lunch
13:00 Hands-on imaging: Multiphoton image acquisition in live ex vivo tissues and tumors
15:00 Coffee Break
15:30 Image analysis: 4D image reconstruction and cell tracking using Imaris and FIJI/ImageJ
17:00 Break
17:30 Evening lecture: Developing radiotracers for translational immunoimaging
18:30 Dinner on site
19:00 Hands-on focus sessions (voluntarily)
Wednesday
09:00 Morning lecture: Multiplexed tissue analysis by CODEX
10:00 ‘Hands-on theory’ at the microscope: Principles of tissue clearing and light-sheet microscopy (LSM)
12:00 Lunch in town
13:00 Hands-on imaging: Image acquisition of whole-organ and whole-mouse samples
15:00 Coffee Break
15:30 Image analysis: Stitching, segmentation & quantification using Imaris and SyGlass VR
17:00 Break
17:30 Evening lecture: Multiparametric image analysis
18:00 Dinner on site
19:00 Hands-on focus sessions (voluntarily)
Thursday
09:00 Morning lecture: Metabolic immuno-MRI
10:00 ‘Hands-on theory’ at the MR: Monitoring immunotherapy response by functional MR & correlated LSM
12:00 Lunch
13:00 Hands-on imaging: MR imaging of ex vivo samples for MR-LSM coregistration
15:00 Coffee Break
15:30 Image analysis: Coregistration of MR-LSM images, considerations for multimodal image quantification
17:00 Break
17:30 Pub Quiz and Closing Dinner in Town
Friday
09:00 ‘Hands-on theory’ at the PET/CT: Tracking immune cells by PET/CT and correlated microscopic imaging
12:00 Lunch
13:00 Hands-on generation of 3D – 2D datasets: Linking PET/CT and whole-mouse LSM with 2D tissue analysis
14:00 Break
14:30 Image analysis: 3D to 2D coregistration for correlated multiplexed tissue analysis
16:00 Wrap-up Coffee and Goodbye
Registration
Please find the registration form here
Please send your filled registration form to: Multiscale-Immunoimaging( at )med.uni-tuebingen.de
Early bird rate: 1.100 Euro / 1.300 Euro / 2.000 Euro
Registration by November 01, 2024
Payment by November 15, 2024
Regular rate: 1.300 Euro / 1.600 Euro / 2.300 Euro
Registration after November 15, 2024
Payment due on January 24, 2024
Rates are listed for
students / regular attendees (academic institutes) / companies.
The seat for the workshop is only guaranteed if payment or at least proof of payment is received no later than the due date specified for the individual rates.
Your registration will include:
Handout materials, lunch, dinner and coffee breaks for all workshop days, all costs for contrast agents, samples, etc.
Methods of Payment:
• Credit card
• Wire transfer
Cancellation fees:
Cancellation by December 13, 2024: 150 € + potential costs for transfer/wiring
Cancellation after December 13, 2024: 250 € + potential costs for transfer/wiring
Cancellation after January 24, 2025: The full registration fee will be due
In case of any question, please feel free to contact us. We will find a suitable solution for all.
Event Details
The entire workshop will be held in the laboratories and lecture halls of the Werner Siemens Imaging Center.
Please note that large portions of the workshop are held in radiation safety areas, where access for pregnant women is not permitted!
Directions:
We are located at
Werner Siemens Imaging Center
Röntgenweg 11 & 13
72076 Tübingen
Building 312 & 315
Find us via "Navi@UKT": https://www.medizin.uni-tuebingen.de/go/anreise inside the clinic campus.
Please klick on the link "Navi@UKT", follow the instructions and use the navigation app.
You may also use the QR on the left.
See you there!